Determination of Lupeol, a Cytotoxic Compound Against SW620 Cells in the Extracts of Ha-Rak Recipe

Introduction: Ha-Rak is an herbal recipe that has been stated by Ministry of Public Health, Thailand to utilize as the medicines for treatment fever. The comprises roots five plants including Capparis micracantha, Clerodendrum indicum, Ficus racemosa, Harrisonia perforata and Tiliacora triandra its ingredients. Methods: extracts principles were evaluated their potential on cytotoxicity against SW620 cancer cell lines using MTT assay active compound, lupeol which was found a cytotoxic compound in ingredients further determined. A chromatographic method investigating developed herein. analytical comprised cholesterol-coated C18 column, mixture acetonitrile methanol (70:30) with flow rate 2 mL/min, diode-array UV wavelength at 210 nm stationary phase, mobile detector, respectively. Results: amount principles, possessed IC50 values 30.10-212.24 ?g/mL displayed variously range 4.50-250.62 mg/100g. difference contents among noted significantly (P < 0.05). correlation between performances tested observed our investigation. Conclusion: Our findings demonstrated contains promising it will support subject pharmacological studies anticancer activity.